Which stage is the second stage of cell division where chromosomes become attached to the spindle fibers?

• A. Metaphase
• B. Prophase
• C. Anaphase
• D. Telophase

Answer: (A)

Metaphase is the stage where the spindle apparatus is fully formed and the chromosomes become attached to spindle fibers from opposite poles, aligning along the cell’s middle. This arrangement, with kinetochores connected to microtubules, ensures the sister chromatids are held in place and pulled apart accurately in the next stage. Earlier in prophase the chromosomes condense and the spindle starts to form, but the crucial attachments to the spindle fibers are established as the cells reach metaphase, preparing for separation in the following stage. Telophase and the subsequent steps involve re-forming nuclei and pulling chromosomes apart, not the attachment and alignment that occur in metaphase.